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KMID : 1140120080130040292
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Cancer Prevention Research
2008 Volume.13 No. 4 p.292 ~ p.296
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Possible Relationship between AMPK and Apoptosis in HT-29 Colon Cancer Cells Treated with CLA
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Park Song-Yi
Lee Yun-Kyoung
Lee Won-Sup
Park Ock-Jin
Kim Young-Min
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Abstract
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Conjugated linoleic acid (CLA), an essential fatty acid linoleic acids (LA), has been shown to suppress cancer cell proliferation and induce apoptosis in several cancer cells. AMP-activated protein kinase (AMPK), a highly conserved protein in eukaryotes, function as an intrinsic regulator of mammalian cell cycle and primarily as a sensor of cellular energy level. In this study, we have examined the effect of CLA on apoptosis and the regulatory effect of AMPK and cyclooxygenase-2 (COX-2) pathway in HT-29 colon cancer. Treatment of CLA (20∼320μM) for 24 h inhibited proliferation of cancer cells and increased chromatin condensation levels represented apoptotic death. These apoptotic effects seemed to be related to AMPK-COX-2 pathways. CLA strongly activated AMPK at the concentration of 40μM and above and in the same condition, COX-2 expression was decreased in cancer cells. ROS is a possible upstream signal of AMPK. CLA generated reactive oxygen species (ROS) at the concentration of 40μM and 160μM in HT-29 colon cancer cells. These observations suggest that AMPK has an important role in cancer cell apoptosis induced by CLA and further AMPK-COX-2 pathway may be a possible target in cancer control.
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KEYWORD
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CLA (conjugated linoleic acid), HT-29 colon cancer cells, Modulating AMP-activated protein kinase, AMPK-COX-2 pathway
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